Histone H3 (acetyl K14) monoclonal antibody, clone RM130

Catalog # MAB12830

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Price

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Quantity

Size:100 ug
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
ChIP
Application

ChIP

ChIP analysis of HeLa cells with Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.

Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 0.5 ug/mL working concentration.

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) (Red) under 2 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against of human histone H3 (acetyl K14).Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding K14 of human Histone H3.

    Sequence

    N/A

    Specificity

    This antibody reacts to histone H3 acetylated at Lysine 14, and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4, Lysine 9, Lysine 18, Lysine 23, Lysine 27, Lysine 36, or Lysine 79 in histone H3.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ChIP (2-10 ug/mL)
    ELISA (0.2-1 ug/mL)
    Immunocytochemistry (0.5-2 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    ChIP

    ChIP analysis of HeLa cells with Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated.

    Western Blot (Cell lysate)

    Western blot analysis of Lane 1: HeLa cells, Lane 2: HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) under 0.5 ug/mL working concentration.

    Immunocytochemistry

    Immunocytochemistry staining of HeLa cells treated with sodium butyrate using Histone H3 (acetyl K14) monoclonal antibody, clone RM130 (Cat # MAB12830) (Red) under 2 ug/mL working concentration. Actin filaments were stained with fluorescein phalloidin (Green).

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — HIST1H3A

    Entrez GeneID

    8350

    Gene Name

    HIST1H3A

    Gene Alias

    H3/A, H3FA

    Gene Description

    histone cluster 1, H3a

    Omim ID

    602810

    Gene Ontology

    Hyperlink

    Gene Summary

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq

    Other Designations

    H3 histone family, member A|histone 1, H3a

  • Interactomes
  • Pathways
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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