Histone H4 (acetyl K5) monoclonal antibody, clone RM199

Catalog # MAB12785

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Size:100 ug
Price: USD $ 531.00
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  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot
Application

Western Blot

Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (acetyl K5) monoclonal antibody, clone RM199 (Cat # MAB12785) at 0.2 ug/mL working concentration, showed a band of Histone H4 acetylated at Lysine 5.

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Histone H4 (acetyl K5) monoclonal antibody, clone RM199 (Cat# MAB12785) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Histone H4 (acetyl K5) monoclonal antibody, clone RM199 (Cat# MAB12785) specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against of human histone H4 (acetyl K5).Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding K5 of human Histone H4.

    Sequence

    N/A

    Specificity

    This antibody reacts to Histone H4 acetylated at Lysine 5. No cross reactivity with other acetylated Lysines in Histone H4.

    Form

    Liquid

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.2 ug/mL-1 ug/mL)
    Western Blot (0.2 ug/mL-1 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (acetyl K5) monoclonal antibody, clone RM199 (Cat # MAB12785) at 0.2 ug/mL working concentration, showed a band of Histone H4 acetylated at Lysine 5.

    Immunocytochemistry

    Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Histone H4 (acetyl K5) monoclonal antibody, clone RM199 (Cat# MAB12785) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — HIST4H4

    Entrez GeneID

    121504

    Protein Accession#

    P62805

    Gene Name

    HIST4H4

    Gene Alias

    H4/p, MGC24116

    Gene Description

    histone cluster 4, H4

    Gene Ontology

    Hyperlink

    Gene Summary

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq

    Other Designations

    histone 4, H4|histone H4

  • Interactomes
  • Pathways
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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