Mouse IgM monoclonal antibody, clone RM109
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgM monoclonal antibody, clone RM109 (Cat # MAB12772) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgM.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgM monoclonal antibody, clone RM109 (Cat # MAB12772) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Igh-6
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