Mouse IgG1 monoclonal antibody, clone RM106
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG1 (20 ng/lane) with Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1 (~150 kDa) stronger than the reduced gamma 1 form (~50 kDa).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Mouse
Specificity
This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (0.005 - 0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5 - 2 ug/mL)
Immunohistochemistry (0.5 - 2 ug/mL)
Immunoprecipitation
Western Blot (0.1 - 0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG1 (20 ng/lane) with Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1 (~150 kDa) stronger than the reduced gamma 1 form (~50 kDa).Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG1 monoclonal antibody, clone RM106 (Cat # MAB12766) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
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Gene Info — Ighg1
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