MYD88 monoclonal antibody, clone AT22F11
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MYD88.
Immunogen
Recombinant protein corresponding to amino acids 1-309 of human MYD88.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Raji cell lysate (40 ug) by using MYD88 monoclonal antibody, clone AT22F11 (Cat # MAB11213) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of MYD88 in A-549 cells. The cell was stained with MYD88 monoclonal antibody, clone AT22F11 (Cat # MAB11213) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of MYD88 in A-549 cell line, staining at 2-5 ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — MYD88
Entrez GeneID
4615Protein Accession#
NP_002459; Q99836Gene Name
MYD88
Gene Alias
MYD88D
Gene Description
myeloid differentiation primary response gene (88)
Omim ID
602170Gene Ontology
HyperlinkOther Designations
myeloid differentiation primary response gene 88
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Interactome
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Disease
- Arthritis
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