LYVE1 monoclonal antibody, clone 4G1
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant LYVE1.
Immunogen
Recombinant protein corresponding to amino acids 25-235 of human LYVE1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-549 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of A549 cells line, stained with DAPI (Blue) for nucleus staining and LYVE1 monoclonal antibody , clone 4G1 (1 : 500) (Cat # MAB1067) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — LYVE1
Entrez GeneID
10894GeneBank Accession#
NM_006691Protein Accession#
NP_006682Gene Name
LYVE1
Gene Alias
CRSBP-1, HAR, LYVE-1, XLKD1
Gene Description
lymphatic vessel endothelial hyaluronan receptor 1
Omim ID
605702Gene Ontology
HyperlinkGene Summary
This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq
Other Designations
cell surface retention sequence binding protein-1|extracellular link domain containing 1|extracellular link domain-containing 1|hyaluronic acid receptor
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Interactome
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Disease
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Publication Reference
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Novel expression and characterization of lymphatic vessel endothelial hyaluronate receptor 1 (LYVE-1) by conjunctival cells.
Chen L, Cursiefen C, Barabino S, Zhang Q, Dana MR.
Investigative Ophthalmology & Visual Science 2005 Dec; 46(12):4536.
Application:IF, IHC-Fr, Mouse, Mouse eyes.
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LYVE-1 immunohistochemical assessment of lymphangiogenesis in endometrial and lung cancer.
Koukourakis MI, Giatromanolaki A, Sivridis E, Simopoulos C, Gatter KC, Harris AL, Jackson DG.
Journal of Clinical Pathology 2005 Feb; 58(2):202.
Application:IHC-P, Human, Human endometrial adenocarcinoma.
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LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.
Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG.
The Journal of Cell Biology 1999 Feb; 144(4):789.
Application:IF, IHC-P, WB-Tr, Monkey, COS-1 cells, HUVECs, Human tissues.
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Novel expression and characterization of lymphatic vessel endothelial hyaluronate receptor 1 (LYVE-1) by conjunctival cells.
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