KDR monoclonal antibody, clone 4B4
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) against extracellular domain of human KDR (aa 20-764) .
Immunofluorescence
Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) (green) . Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) (green) and negative control (purple).
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant KDR.
Immunogen
Recombinant hIgGFc tag fusion protein corresponding to extracellular fragment at amino acids 20-764 of human KDR.
Host
Mouse
Theoretical MW (kDa)
152
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) against extracellular domain of human KDR (aa 20-764) .Immunofluorescence
Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells using KDR monoclonal antibody, clone 4B4 (Cat # MAB10584) (green) and negative control (purple). -
Gene Info — KDR
Entrez GeneID
3791Gene Name
KDR
Gene Alias
CD309, FLK1, VEGFR, VEGFR2
Gene Description
kinase insert domain receptor (a type III receptor tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
Other Designations
soluble VEGFR2|vascular endothelial growth factor receptor 2
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Interactomes
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Pathways
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Diseases
- Adenocarcinoma
- Alzheimer disease
- Angina Pectoris
- Anoxia
- Asthma
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
+ View More Disease
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