PLA2G12A monoclonal antibody (M01), clone 1D11
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PLA2G12A.
Immunogen
PLA2G12A (NP_110448, 90 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICRDVQKTLGLTQHVQACETTVELLFDSVIHLGCKPYLDSQRAACRCHYEEKTD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLA2G12A expression in transfected 293T cell line by PLA2G12A monoclonal antibody (M01), clone 1D11.
Lane 1: PLA2G12A transfected lysate(21.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLA2G12A is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — PLA2G12A
Entrez GeneID
81579GeneBank Accession#
NM_030821Protein Accession#
NP_110448Gene Name
PLA2G12A
Gene Alias
GXII, PLA2G12, ROSSY
Gene Description
phospholipase A2, group XIIA
Omim ID
611652Gene Ontology
HyperlinkGene Summary
Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM
Other Designations
group XII secreted phospholipase A2|group XIIA secreted phospholipase A2
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of group XIIA phospholipase A2 in human digestive organs.
Peuravuori H, Kollanus S, Nevalainen TJ.
APMIS : Acta Pathologica, Microbiologica, et Immunologica Scandinavica 2014 Dec; 122(12):117.
Application:IHC-P, Human, Oesophagus, Stomach, Small intestine, Large intestine, Gall bladder, Pancreas.
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Expression of group XIIA phospholipase A2 in human digestive organs.
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