HSPA2 monoclonal antibody (M06), clone S51
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant HSPA2.
Immunogen
HSPA2 (AAH01752, 1 a.a. ~ 639 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFGDATVQSDMKHWPFRVVSEGGKPKVQVEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKVHSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEEVD
Host
Mouse
Reactivity
Human
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (96.03 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HSPA2 monoclonal antibody (M06), clone S51. Western Blot analysis of HSPA2 expression in HeLa(Cat # L013V1 ).Western Blot (Cell lysate)
HSPA2 monoclonal antibody (M06), clone S51. Western Blot analysis of HSPA2 expression in different cell lines.Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSPA2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]Immunoprecipitation
Immunoprecipitation of HSPA2 transfected lysate using anti-HSPA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPA2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSPA2 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — HSPA2
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis.
Sasaki T, Marcon E, McQuire T, Arai Y, Moens PB, Okada H.
Journal of Cellular Biology 2008 Aug; 182(3):449.
Application:IF, IHC-P, Mouse, Mouse testis.
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Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis.
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