BIRC4 monoclonal antibody (M04), clone 1F4
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant BIRC4.
Immunogen
BIRC4 (AAH32729, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDIS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged XIAP is 0.03 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between AKT1 and XIAP. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to XIAP on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — XIAP
Entrez GeneID
331GeneBank Accession#
BC032729Protein Accession#
AAH32729Gene Name
XIAP
Gene Alias
API3, BIRC4, ILP1, MIHA, XLP2
Gene Description
X-linked inhibitor of apoptosis
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq
Other Designations
OTTHUMP00000023975|OTTHUMP00000196392|apoptosis inhibitor 3|baculoviral IAP repeat-containing 4|baculoviral IAP repeat-containing protein 4
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Interactome
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Pathway
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Disease
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