PML/RARA DY Translocation FISH Probe

Catalog # FT0004

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Size:100 uL
Price: USD $ 1,288.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Fluorescent <i>In Situ</i> Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Application

Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)

Human lymph node (FFPE) stained with PML/RARA DY Translocation FISH Probe. Human lymph node cancer showed no PML/RARA DY Translocation.

QC Test

<br>Hybridization position of the probes on the chromosome.
Note


Hybridization position of the probes on the chromosome.


Hybridization position of the probes on the chromosome.

  • Specification

    Product Description

    Labeled FISH probes for identification of gene translocation using Fluorescent In Situ Hybridization Technique. (Technology)

    Probe 1

    Name: RARA
    Size: Approximately 1200kb
    Fluorophore: FITC
    Location: 17q21.2

    Probe 2

    Name: PML
    Size: Approximately 540kb
    Fluorophore: Texas Red
    Location: 15q24.1

    Reactivity

    Human

    Form

    Liquid

    Notice

    We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

    Regulation Status

    For research use only (RUO)

    Quality Control Testing

    Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.

    Supplied Product

    DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe

    Storage Instruction

    Store at 4°C in the dark.

    Note


    Hybridization position of the probes on the chromosome.

  • Applications

    Fluorescent In Situ Hybridization (Cell)

    Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)

    Human lymph node (FFPE) stained with PML/RARA DY Translocation FISH Probe. Human lymph node cancer showed no PML/RARA DY Translocation.
  • Gene Info — PML

    Entrez GeneID

    5371

    Gene Name

    PML

    Gene Alias

    MYL, PP8675, RNF71, TRIM19

    Gene Description

    promyelocytic leukemia

    Omim ID

    102578

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq

    Other Designations

    promyelocytic leukemia protein|promyelocytic leukemia, inducer of|tripartite motif protein TRIM19

  • Gene Info — RARA

    Entrez GeneID

    5914

    Gene Name

    RARA

    Gene Alias

    NR1B1, RAR

    Gene Description

    retinoic acid receptor, alpha

    Omim ID

    180240

    Gene Ontology

    Hyperlink

    Gene Summary

    Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM

    Other Designations

    OTTHUMP00000164454|OTTHUMP00000164456|Retinoic acid receptor, alpha polypeptide|nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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