Fluorescence In Situ Hybridization (FISH)

FISH is a technique used to identify and localize the presence or absence of specific DNA sequences in cells and tissues. Abnova has developed a range of FISH probes for the detection of gene amplification, loss, and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originating from a bacterial artificial chromosome (BAC) library. We continue to expand the scope of the FISH probes to meet the customer’s research needs. Custom probes are also available upon request.

Advantages

Dual-colored probes for fast, sensitive, and specific detection
Work on metaphase spread, paraffin-embedded and frozen tissue
Identify gene amplification, loss, and translocation
High signal-to-noise ratio
Low cross-reactivity

Product Lines

Gene Amplification / Gene Loss

Split Dual Color FISH Probe

Translocation Dual Color FISH Probe

Procedure

Publication Reference

DNA sequence and analysis of human chromosome 8
Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, and et al. Nature. 2006 Jan 19;439(7074):331-5.

Initial sequencing and analysis of the human genome
Lander ES, Linton LM, Birren B, Nusbaum C, and et al. Nature. 2001 Feb 15;409(6822):860-921.

A physical map of the human genome.
McPherson JD, Marra M, Hillier L, Waterston RH, and et al. Nature. 2001 Feb 15;409(6822):934-41.

Human BAC library: construction and rapid screening.
Asakawa S, Abe I, Kudoh Y, Kishi N, Wang Y, Kubota R, Kudoh J, Kawasaki K, Minoshima S, Shimizu N. Gene. 1997 May 20;191(1):69-79.

For any inquiry, please contact : technicalsupport@abnova.com

Technologies