bcr/abl DY Translocation FISH Probe
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Specifications
Product Description
Labeled FISH probes for identification of gene translocation using Fluorescent In Situ Hybridization Technique. (Technology).
Probe 1
Name: BCR
Size: Approximately 1520kb
Fluorophore: FITC
Location: 22q11.23Probe 2
Name: ABL
Size: Approximately 780kb
Fluorophore: Texas Red
Location: 9q34.1Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome. -
Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human CML (FFPE) stained with bcr/abl DY Translocation FISH Probe. Human CML showed bcr/abl gene translocation.Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lymph node (FFPE) stained with bcr/abl DY Translocation FISH Probe. Human lymph node cancer showed no bcr/abl DY Translocation. -
Gene Info — ABL1
Entrez GeneID
25Gene Name
ABL1
Gene Alias
ABL, JTK7, bcr/abl, c-ABL, p150, v-abl
Gene Description
c-abl oncogene 1, receptor tyrosine kinase
Omim ID
189980Gene Ontology
HyperlinkGene Summary
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
Other Designations
Abelson murine leukemia viral (v-abl) oncogene homolog 1|OTTHUMP00000022375|OTTHUMP00000022376|bcr/c-abl oncogene protein|proto-oncogene tyrosine-protein kinase ABL1|v-abl Abelson murine leukemia viral oncogene homolog 1
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Gene Info — BCR
Entrez GeneID
613Gene Name
BCR
Gene Alias
ALL, BCR-ABL1, BCR1, CML, D22S11, D22S662, FLJ16453, PHL
Gene Description
breakpoint cluster region
Gene Ontology
HyperlinkGene Summary
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
bcr-abl1 e14a3 chimeric protein
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Interactomes
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Pathways
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