TOP2A/CEN17q FISH Probe

Catalog # FG0007

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Size:200 uL
Price: USD $ 2,122.00
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Size:100 uL
Price: USD $ 1,288.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Fluorescent <i>In Situ</i> Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Application

Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)

Human breast cancer (FFPE) stained with TOP2A/CEN17q FISH Probe. Human breast cancer showed no TOP2A gene amplification.

QC Test

<br>Hybridization position of the probes on the chromosome.
Note


Hybridization position of the probes on the chromosome.


Hybridization position of the probes on the chromosome.

  • Specification

    Product Description

    Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology)

    Probe 1

    Name: TOP2A
    Size: Approximately 180kb
    Fluorophore: Texas Red
    Location: 17q21

    Probe 2

    Name: CEN17q
    Size: Approximately 540kb
    Fluorophore: FITC
    Location: 17q11.2

    Probe Gap

    The gap between two probes is approximately 6,400 kb.

    Origin

    Human

    Source

    Genomic DNA

    Reactivity

    Human

    Form

    Liquid

    Notice

    We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

    Regulation Status

    For research use only (RUO)

    Quality Control Testing

    Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.

    Supplied Product

    DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe

    Storage Instruction

    Store at 4°C in the dark.

    Note


    Hybridization position of the probes on the chromosome.

  • Applications

    Fluorescent In Situ Hybridization (Cell)

    Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)

    Human breast cancer (FFPE) stained with TOP2A/CEN17q FISH Probe. Human breast cancer showed no TOP2A gene amplification.
  • Gene Info — TOP2A

    Entrez GeneID

    7153

    Gene Name

    TOP2A

    Gene Alias

    TOP2, TP2A

    Gene Description

    topoisomerase (DNA) II alpha 170kDa

    Omim ID

    126430

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq

    Other Designations

    DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha (170kD)

  • Interactome
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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