Products

Product Description

This protein phosphorylation antibody pair set comes with two antibodies, one against the DNMT1 protein, and the other against the specific Y399 phosphorylated site of DNMT1 for use in in situ Proximity Ligation Assay. See Publication Reference below.

Reactivity

Human

Quality Control Testing

Dual recognition immunofluorescence result.

Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

Supplied Product

Antibody pair set content:
1. Phospho-DNMT1 Y399 rabbit polyclonal antibody (20 ul)
In PBS (0.09% (w/v) sodium azide)
2. DNMT1 mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.

Storage Instruction

Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.