HSP90AB1 & GNAI2 Protein Protein Interaction Antibody Pair
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More Files
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Representative image of Proximity Ligation Assay of protein-protein interactions between HSP90AB1 and GNAI2. HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal antibody 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the HSP90AB1 protein, and the other against the GNAI2 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between HSP90AB1 and GNAI2. HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal antibody 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. HSP90AB1 rabbit purified polyclonal antibody (100 ug)
2. GNAI2 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — GNAI2
Entrez GeneID
2771Gene Name
GNAI2
Gene Alias
GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Gene Description
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
GTP-binding regulatory protein Gi alpha-2 chain|WUGSC:H_LUCA15.1|WUGSC:H_LUCA16.1
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Gene Info — HSP90AB1
Entrez GeneID
3326Gene Name
HSP90AB1
Gene Alias
D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB
Gene Description
heat shock protein 90kDa alpha (cytosolic), class B member 1
Omim ID
140572Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta
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Interactome
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Pathway
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Disease
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