CDX1 recombinant monoclonal antibody, clone 11E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 whole cell lysate with CDX1 recombinant monoclonal antibody, clone 11E10 (Cat # RAB07582).
Immunofluorescence
Immunofluorescent staining of Hela Cells with CDX1 recombinant monoclonal antibody, clone 11E10 (Cat # RAB07582), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 524-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDX1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CDX1.
Theoretical MW (kDa)
Calculated MW: 29, 1
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 whole cell lysate with CDX1 recombinant monoclonal antibody, clone 11E10 (Cat # RAB07582).Immunofluorescence
Immunofluorescent staining of Hela Cells with CDX1 recombinant monoclonal antibody, clone 11E10 (Cat # RAB07582), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 524-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDX1
Entrez GeneID
1044Protein Accession#
P47902Gene Name
CDX1
Gene Alias
MGC116915
Gene Description
caudal type homeobox 1
Omim ID
600746Gene Ontology
HyperlinkGene Summary
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity. [provided by RefSeq
Other Designations
caudal type homeo box transcription factor 1|caudal type homeobox transcription factor 1|caudal-type homeobox protein CDX1
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