ENTPD1 recombinant monoclonal antibody, clone 12D12
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More Files
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Flow Cytometry
Flow cytometry shows PC3 cells stained with ENTPD1 recombinant monoclonal antibody, clone 12D12 (Cat # RAB07566)(red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ENTPD1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ENTPD1.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry shows PC3 cells stained with ENTPD1 recombinant monoclonal antibody, clone 12D12 (Cat # RAB07566)(red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. -
Gene Info — ENTPD1
Entrez GeneID
953Protein Accession#
P49961Gene Name
ENTPD1
Gene Alias
ATPDase, CD39, DKFZp686D194, DKFZp686I093, FLJ40921, FLJ40959, NTPDase-1
Gene Description
ectonucleoside triphosphate diphosphohydrolase 1
Omim ID
601752Gene Ontology
HyperlinkOther Designations
CD39 antigen|OTTHUMP00000020161|ecto-ATP diphosphohydrolase|ecto-apyrase|lymphoid cell activation antigen
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Interactome
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Pathway
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Disease
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