GABRA5 recombinant monoclonal antibody, clone 5C11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Ntera-2 whole cell lysate, HEK293 whole cell lysate with GABRA5 recombinant monoclonal antibody, clone 5C11 (Cat # RAB07448).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using GABRA5 recombinant monoclonal antibody, clone 5C11 (Cat # RAB07448) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GABRA5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GABRA5.
Theoretical MW (kDa)
Calculated MW: 53
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Ntera-2 whole cell lysate, HEK293 whole cell lysate with GABRA5 recombinant monoclonal antibody, clone 5C11 (Cat # RAB07448).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using GABRA5 recombinant monoclonal antibody, clone 5C11 (Cat # RAB07448) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GABRA5
Entrez GeneID
2558Protein Accession#
P31644Gene Name
GABRA5
Gene Alias
MGC138184
Gene Description
gamma-aminobutyric acid (GABA) A receptor, alpha 5
Omim ID
137142Gene Ontology
HyperlinkGene Summary
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq
Other Designations
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Pathway
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Disease
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