Mouse IgG3 Fc Gamma fragment VHH recombinant monoclonal antibody (Biotin)
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Specification
Product Description
Alpaca VHH recombinant monoclonal antibody raised against Fc region of mouse IgG3.
The antibody is conjugated with biotin.Antibody Species
Alpaca
Immunogen
Recombinant Fc region of mouse IgG3
Reactivity
Mouse
Specificity
Mouse IgG3 (Fcγ fragment specific)
Form
Liquid
Conjugation
Biotin
Purification
Protein A
Isotype
Alpaca IgG2b/2c
Recommend Usage
ELISA (1:5000-1:20000)
Immunoprecipitation (1-2ug)
Surface Plasmon Resonance
Western Blot (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.5 (0.05% sucrose, 0.1% trehalose, 0.01% proclin 300)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. Mouse VHH IgG3 Fc Gamma recombinant monoclonal antibody (Biotin) (Cat # RAB07109) was used as the primary antibody at 1:10000 and streptavidin (HRP) (Cat # RAB07268) as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. Mouse VHH IgG3 Fc Gamma recombinant monoclonal antibody (Biotin) (Cat # RAB07109) was used as the primary antibody at 1:1000 and streptavidin (HRP) (Cat # RAB07268) as the secondary antibody.Surface Plasmon Resonance
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