FBXO5 recombinant monoclonal antibody, clone R02-1H4
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Emi1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Emi1
Theoretical MW (kDa)
Calculated MW: 50 kD
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Immunofluorescence (1/50-1/200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1/50-1/100)
Immunoprecipitation (1/20)
Western Blot (1/500-1/1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — FBXO5
Entrez GeneID
26271Gene Name
FBXO5
Gene Alias
EMI1, FBX5, Fbxo31
Gene Description
F-box protein 5
Omim ID
606013Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
F-box only protein 5|F-box protein Fbx5|OTTHUMP00000017453|early mitotic inhibitor 1
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