PABPN1 recombinant monoclonal antibody, clone R02-8B1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat, Hela, COS7, Raji lysates with PABPN1 recombinant monoclonal antibody, clone R02-8B1 (Cat # RAB04903).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human breast cancer using PABPN1 recombinant monoclonal antibody, clone R02-8B1 (Cat # RAB04903). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PABPN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PABPN1.
Theoretical MW (kDa)
Calculated MW: 33 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunohistochemistry (Frozen sections) (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat, Hela, COS7, Raji lysates with PABPN1 recombinant monoclonal antibody, clone R02-8B1 (Cat # RAB04903).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human breast cancer using PABPN1 recombinant monoclonal antibody, clone R02-8B1 (Cat # RAB04903). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — PABPN1
Entrez GeneID
8106Protein Accession#
Q86U42Gene Name
PABPN1
Gene Alias
OPMD, PAB2, PABP2
Gene Description
poly(A) binding protein, nuclear 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. An expansion of the trinucleotide (GCG) repeat from normal 6 to 8-13 at the 5' end of the coding region of this gene leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Multiple splice variants have been described but their full-length nature is not known. One splice variant includes introns 1 and 6 but no protein is formed. [provided by RefSeq
Other Designations
poly(A) binding protein 2|poly(A) binding protein II
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Interactome
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Disease
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