GTF2B recombinant monoclonal antibody, clone R06-4C2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 3T3 whole cell lysate and Lane 2: Raw264.7 whole cell lysate with GTF2B recombinant monoclonal antibody, clone R06-4C2 (Cat # RAB04434).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GTF2B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GTF2B.
Theoretical MW (kDa)
Calculated MW: 35 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 3T3 whole cell lysate and Lane 2: Raw264.7 whole cell lysate with GTF2B recombinant monoclonal antibody, clone R06-4C2 (Cat # RAB04434).Immunoprecipitation
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Gene Info — GTF2B
Entrez GeneID
2959Protein Accession#
Q00403Gene Name
GTF2B
Gene Alias
TF2B, TFIIB
Gene Description
general transcription factor IIB
Omim ID
189963Gene Ontology
HyperlinkGene Summary
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq
Other Designations
OTTHUMP00000012347|OTTHUMP00000058838|RNA polymerase II transcription factor IIB
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Interactome
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Pathway
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