ALDOA recombinant monoclonal antibody, clone 7D8
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: NIH/3T3 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: A549 whole cell lysate; Lane 6: Mouse Liver whole cell lysate; Lane 7:Rat Liver whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of ALDOA recombinant monoclonal antibody, clone 7D8 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with ALDOA recombinant monoclonal antibody, clone 7D8 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat ALDOA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human ALDOA.
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: NIH/3T3 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: A549 whole cell lysate; Lane 6: Mouse Liver whole cell lysate; Lane 7:Rat Liver whole cell lysate.Immunohistochemistry
Immunohistochemistry image of ALDOA recombinant monoclonal antibody, clone 7D8 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with ALDOA recombinant monoclonal antibody, clone 7D8 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ALDOA
Entrez GeneID
226Protein Accession#
P04075Gene Name
ALDOA
Gene Alias
ALDA, MGC10942, MGC17716, MGC17767
Gene Description
aldolase A, fructose-bisphosphate
Omim ID
103850Gene Ontology
HyperlinkGene Summary
This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein. [provided by RefSeq
Other Designations
aldolase A|fructose-1,6-bisphosphate triosephosphate-lyase|fructose-bisphosphate aldolase A
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Fructose and mannose metabolism
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