Tnfsf8 recombinant monoclonal antibody, clone RM153
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More Files
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Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Tnfsf8 recombinant monoclonal antibody, clone RM153 (Cat # RAB03162).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03162 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03162- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Tnfsf8.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against the CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
Flow Cytometry
Functional Study
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Tnfsf8 recombinant monoclonal antibody, clone RM153 (Cat # RAB03162).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03162 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03162- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Functional Study
Blocking
Flow Cytometry
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Gene Info — Tnfsf8
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