PIM1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:BT-20 whole cell lysate Lane2:HepG2 whole cell lysate Lane3:SW480 whole cell lysate with PIM1 recombinant monoclonal antibody (Cat # RAB02771) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using PIM1 recombinant monoclonal antibody (Cat # RAB02771).Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of BT-20 cells using PIM1 recombinant monoclonal antibody (Cat # RAB02771)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PIM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PIM1.
Theoretical MW (kDa)
45
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of PIM1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:BT-20 whole cell lysate Lane2:HepG2 whole cell lysate Lane3:SW480 whole cell lysate with PIM1 recombinant monoclonal antibody (Cat # RAB02771) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using PIM1 recombinant monoclonal antibody (Cat # RAB02771).Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of BT-20 cells using PIM1 recombinant monoclonal antibody (Cat # RAB02771)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — PIM1
Entrez GeneID
5292Protein Accession#
P11309Gene Name
PIM1
Gene Alias
PIM
Gene Description
pim-1 oncogene
Omim ID
164960Gene Ontology
HyperlinkGene Summary
The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM
Other Designations
OTTHUMP00000018046|Oncogene PIM1|non-specific serine/threonine protein kinase|pim-1 kinase 44 kDa isoform|pim-1 oncogene (proviral integration site 1)
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Interactome
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Pathway
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Disease
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