14-3-3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat hippocampal (Hipp) lysate showing immunolabeling of the ~29k 14-3-3 protein.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of 14-3-3 protein.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus rat 14-3-3 protein.
Host
Rabbit
Theoretical MW (kDa)
29
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal (Hipp) lysate showing immunolabeling of the ~29k 14-3-3 protein. -
Publication Reference
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14-3-3 proteins: a number of functions for a numbered protein.
Bridges D, Moorhead GB.
Science's STKE : Signal Transduction Knowledge Environment 2005 Aug; 2005(296):re10.
Application：WB, Human, Human mammalian cells .
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14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.
Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T.
The Journal of Biological Chemistry 2004 Nov; 279(47):49113.
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Unlocking the code of 14-3-3.
Dougherty MK, Morrison DK.
Journal of Cell Science 2004 Apr; 117(Pt 10):1875.
Application：WB, Human, Human mammalian cells.
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14-3-3 proteins: a number of functions for a numbered protein.
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