MKRN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with MKRN1 polycolnal antibody (Cat # PAB27575) (0.3ug/ml) in Lane 1 and probed with anti- DYKDDDDK Tag (1/1000) in Lane 3.
Lane 2: Mock-transfected HEK293 probed with MKRN1 polycolnal antibody (Cat # PAB27575) (1mg/ml). Primary incubations were for 1 hour. Detected by
chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MKRN1.
Immunogen
A synthetic peptide corresponding to amino acids 105-118 at internal region of human MKRN1.
Sequence
RYEHSKPLKQEEAT
Host
Goat
Theoretical MW (kDa)
60
Reactivity
Chicken, Dog, Human, Mouse, Pig
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with MKRN1 polycolnal antibody (Cat # PAB27575) (0.3ug/ml) in Lane 1 and probed with anti- DYKDDDDK Tag (1/1000) in Lane 3.
Lane 2: Mock-transfected HEK293 probed with MKRN1 polycolnal antibody (Cat # PAB27575) (1mg/ml). Primary incubations were for 1 hour. Detected by chemiluminescence. -
Gene Info — MKRN1
Entrez GeneID
23608Protein Accession#
NP_038474.2;NP_001138597.1Gene Name
MKRN1
Gene Alias
FLJ21334, RNF61
Gene Description
makorin ring finger protein 1
Omim ID
607754Gene Ontology
HyperlinkGene Summary
The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000 [PubMed 10843807]).[supplied by OMIM
Other Designations
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Interactome
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Disease
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