STAT3 (phospho Y705) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
The whole cell lysate derived from A-431 with EGF stimulated (Lane 1) or without EGF stimulated (Lane 2) were immunoblotted by STAT3 (phospho Y705) polyclonal antibody (Cat # PAB12754) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pY705, Phospho-specific).
B : STAT3 (Paired Y705, Non phosphospeicfic).
Followed were blotted at a 1 : 2000 dilution by :
1. STAT3 (phospho Y705) polyclonal antibody (Cat # PAB12754).
2. Rabbit anti-STAT3 (Paired Y705).
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y705 of human, mouse, rat, bovine, chicken and dog STAT3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody recognizes STAT3 with a phosphorylated sites of Tyr705. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Purification
Site-modified Epitope Affinity Purification
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (1:50)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for three month without detectable loss of activity. For long term storage, store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from A-431 with EGF stimulated (Lane 1) or without EGF stimulated (Lane 2) were immunoblotted by STAT3 (phospho Y705) polyclonal antibody (Cat # PAB12754) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : STAT3 (pY705, Phospho-specific).
B : STAT3 (Paired Y705, Non phosphospeicfic).
Followed were blotted at a 1 : 2000 dilution by :
1. STAT3 (phospho Y705) polyclonal antibody (Cat # PAB12754).
2. Rabbit anti-STAT3 (Paired Y705). -
Publication Reference
-
A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
Matsuo R, Ochiai W, Nakashima K, Taga T.
Journal of Immunology 2001 Jan; 247(1-2):141.
-
A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com