Goat Anti-Human IgG F(c) secondary antibody (Fluorescein)
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Specifications
Product Description
Goat anti-human IgG recognizes human IgG F(c) fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Human IgG F(c) fragment
Host
Goat
Reactivity
Human
Form
Liquid
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
2.9 moles FITC per mole of goat IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Covalent immobilisation of VEGF on plasma-coated electrospun scaffolds for tissue engineering applications.
Guex AG, Hegemann D, Giraud MN, Tevaearai HT, Popa AM, Rossi RM, Fortunato G.
Colloids and Surfaces. B, Biointerfaces 2014 Nov; 123:724.
Application：IF, Human, Endothelial cells.
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
The TH, Feltkamp TE.
Immunology 1970 Jun; 18(6):865.
Application：Conjugation, Fluorescein isothiocyanate (FITC).
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Covalent immobilisation of VEGF on plasma-coated electrospun scaffolds for tissue engineering applications.
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