SPARC (Human) Recombinant Protein
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Specification
Product Description
Human SPARC (P09486, Ala18-Ile303) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Ala18-Ile303
Host
Human
Theoretical MW (kDa)
33.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.10 ug/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human SPARC is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human SPARC on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human SPARC, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — SPARC
Entrez GeneID
6678Protein Accession#
P09486Gene Name
SPARC
Gene Alias
ON
Gene Description
secreted protein, acidic, cysteine-rich (osteonectin)
Omim ID
182120Gene Ontology
HyperlinkGene Summary
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM
Other Designations
cysteine-rich protein|osteonectin|secreted protein, acidic, cysteine-rich
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Interactome
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