PSCA (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PSCA, hFc Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-PSCA Antibody, hFc Tag with the EC50 of 117.3 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human PSCA-is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human PSCA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human PSCA (O43653, Leu12-Ser86) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Leu12-Ser86
Host
Human
Theoretical MW (kDa)
34.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 117.3 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human PSCA-is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human PSCA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PSCA, hFc Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-PSCA Antibody, hFc Tag with the EC50 of 117.3 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — PSCA
Entrez GeneID
8000Protein Accession#
O43653Gene Name
PSCA
Gene Alias
PRO232
Gene Description
prostate stem cell antigen
Omim ID
602470Gene Ontology
HyperlinkGene Summary
This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene has a nonsynonymous nucleotide polymorphism at its start codon. [provided by RefSeq
Other Designations
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Interactome
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Disease
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