CR2 (Human) Recombinant Protein
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Specification
Product Description
Human CR2 (P20023-1, Ile21-Arg971) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Ile21-Arg971
Host
Human
Theoretical MW (kDa)
105.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 7.9 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD21 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD21, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CR2
Entrez GeneID
1380Protein Accession#
P20023-1Gene Name
CR2
Gene Alias
C3DR, CD21, SLEB9
Gene Description
complement component (3d/Epstein Barr virus) receptor 2
Gene Ontology
HyperlinkGene Summary
Complement component receptor-2 (CR2) is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. See also CR1 (MIM 120620). Yefenof et al. (1976) [PubMed 181330] found complete overlapping of EBV receptors and C3 (MIM 120700) receptors on human B lymphocytes.[supplied by OMIM
Other Designations
OTTHUMP00000034421|OTTHUMP00000034447
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Interactome
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