HPN (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human HPN, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 10.4 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human HPN is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HPN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human HPN (P05981, Arg45-Leu417) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Arg45-Leu417
Host
Human
Theoretical MW (kDa)
42
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 10.4 ng/mL, messured by ELISA at 2 ug/mL.
Isotype
PGF (Human) Recombinant Protein
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human HPN is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HPN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HPN, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 10.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — HPN
Entrez GeneID
3249Protein Accession#
P05981Gene Name
HPN
Gene Alias
TMPRSS1
Gene Description
hepsin
Omim ID
142440Gene Ontology
HyperlinkGene Summary
This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants
Other Designations
transmembrane protease, serine 1
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Interactome
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Disease
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