HLA-A/B2M/GP100 (YLEPGPVTA) Tetramer (Human) Recombinant Protein
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Specification
Product Description
Human HLA-A/B2M/GP100 (YLEPGPVTA) Tetramer (A0A140T913/P61769/P04637, Gly25-Thr305/Ile21-Met119/YLEPGPVTA) partial recombinant protein with His-Avi tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly25-Thr305;Ile21-Met119;YLEPGPVTA peptide
Host
Human
Theoretical MW (kDa)
258
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 13.4 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). -
Gene Info — B2M
Entrez GeneID
567Protein Accession#
A0A140T913;P61769;YLEPGPVTAGene Name
B2M
Gene Alias
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Gene Description
beta-2-microglobulin
Gene Ontology
HyperlinkGene Summary
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
Other Designations
beta chain of MHC class I molecules|beta-2-microglobin
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Gene Info — HLA-A
Entrez GeneID
3105Protein Accession#
A0A140T913;P61769;YLEPGPVTAGene Name
HLA-A
Gene Alias
HLAA
Gene Description
major histocompatibility complex, class I, A
Gene Ontology
HyperlinkGene Summary
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, A-23 alpha chain|MHC class I antigen HLA-A heavy chain|MHC leukocyte antigen|OTTHUMP00000161059|antigen presenting molecule|leucocyte antigen class I|leukocyte antigen class I-A
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Gene Info — SILV
Entrez GeneID
6490Protein Accession#
A0A140T913;P61769;YLEPGPVTAGene Name
SILV
Gene Alias
D12S53E, ME20, PMEL, PMEL17, SI, SIL, gp100
Gene Description
silver homolog (mouse)
Omim ID
155550Gene Ontology
HyperlinkGene Summary
mouse
Other Designations
Melanocyte protein mel 17|Pmel 17|Silver, mouse, homolog of|melanosomal matrix protein17|silver (mouse homolog)-like|silver homolog
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