HLA-A/B2M/MAGEA4 Tetramer (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 6.1 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Anti-HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49 pM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
-
Specification
Product Description
Human HLA-A | B2M | MAGEA4 Tetramer (A0A140T913|P61769|P43358, Gly25-Thr305 | Ile21-Met119 | GVYDGREHTV) partial recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
Sequence
Gly25-Thr305 | Ile21-Met119 | GVYDGREHTV
Host
Human
Theoretical MW (kDa)
258
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 6.1 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Anti-HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01 & B2M & MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49 pM as determined in SPR assay (Biacore T200). -
Gene Info — B2M
Entrez GeneID
567Protein Accession#
A0A140T913(HLA-A*02:01) | P61769(B2M) | GVYDGREHTVGene Name
B2M
Gene Alias
-
Gene Description
beta-2-microglobulin
Gene Ontology
HyperlinkGene Summary
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
Other Designations
beta chain of MHC class I molecules|beta-2-microglobin
-
Gene Info — MAGEA4
Entrez GeneID
4103Protein Accession#
A0A140T913(HLA-A*02:01) | P61769(B2M) | GVYDGREHTVGene Name
MAGEA4
Gene Alias
MAGE-41, MAGE-X2, MAGE4, MAGE4A, MAGE4B, MGC21336
Gene Description
melanoma antigen family A, 4
Omim ID
300175Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000025889|OTTHUMP00000025890|melanoma-associated antigen 4
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com