MS4A1 (Human) Recombinant Protein
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Specification
Product Description
Human MS4A1 (P11836-1, Met1-Pro297) partial recombinant protein expressed in HEK293 cells.
Sequence
Met1-Pro297
Host
Human
Theoretical MW (kDa)
Predicted MW: 34.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.7 ng/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human CD20 VLP is greater than 95% as determined by SEC-HPLC.
Recommend Usage
Biological Activity
ELISA
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD20 VLP at 5 ug/mL (100 uL/Well) on plate on the plate. Dose response curve for Rituximab, hFc Tag with the EC50 of 8.7 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
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Gene Info — MS4A1
Entrez GeneID
931Protein Accession#
P11836-1Gene Name
MS4A1
Gene Alias
B1, Bp35, CD20, LEU-16, MGC3969, MS4A2, S7
Gene Description
membrane-spanning 4-domains, subfamily A, member 1
Omim ID
112210Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq
Other Designations
B-lymphocyte cell-surface antigen B1|CD20 antigen|CD20 receptor
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Interactome
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Disease
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