IL10RA (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-10, No Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human IL10RA (Q13651, His22-Asn235) partial recombinant protein with His-Avi tag at C-Terminus expressed in HEK293 cells.
Sequence
His22-Asn235
Host
Human
Theoretical MW (kDa)
27
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 21.8 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-10, No Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). -
Gene Info — IL10RA
Entrez GeneID
3587Protein Accession#
Q13651Gene Name
IL10RA
Gene Alias
CDW210A, HIL-10R, IL-10R1, IL10R
Gene Description
interleukin 10 receptor, alpha
Omim ID
146933Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq
Other Designations
-
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com