CLEC5A (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human MDL-1, hFc Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CLEC5A (Q9NY25-1, Tyr26-Lys188) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Tyr26-Lys188
Host
Human
Theoretical MW (kDa)
46
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 17.4 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human MDL-1, hFc Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CLEC5A
Entrez GeneID
23601Protein Accession#
Q9NY25-1Gene Name
CLEC5A
Gene Alias
CLECSF5, MDL-1, MDL1, MGC138304
Gene Description
C-type lectin domain family 5, member A
Omim ID
604987Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq
Other Designations
C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 5|C-type lectin, superfamily member 5|myeloid DAP12-associating lectin-1
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