MPL (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human c-MPL, His Tag at 0.2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human MPL (P40238-1, Gln26-Trp491) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln26-Trp491
Host
Human
Theoretical MW (kDa)
53.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 9.1 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human c-MPL, His Tag at 0.2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
-
Gene Info — MPL
Entrez GeneID
4352Protein Accession#
P40238-1Gene Name
MPL
Gene Alias
C-MPL, CD110, MPLV, TPOR
Gene Description
myeloproliferative leukemia virus oncogene
Gene Ontology
HyperlinkGene Summary
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq
Other Designations
OTTHUMP00000008582|thrombopoietin receptor
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com