IL17RC (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-17A, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 60.6 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human IL-17Rc is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-17Rc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL17RC (NP_703190.2 Leu21-Arg467) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Leu21-Arg467
Host
Human
Theoretical MW (kDa)
76.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 60.6 ng/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-17Rc is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-17Rc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-17A, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 60.6 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — IL17RC
Entrez GeneID
84818Protein Accession#
NP_703190.2Gene Name
IL17RC
Gene Alias
FLJ95963, FLJ96005, IL17-RL, IL17RL, MGC10763
Gene Description
interleukin 17 receptor C
Omim ID
610925Gene Ontology
HyperlinkGene Summary
This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. [provided by RefSeq
Other Designations
IL17F receptor|interleukin 17 receptor-like
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