CADM1 (Human) Recombinant Protein
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Specification
Product Description
Human CADM1 (Q9BY67-1, Gln45-His374) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Gln45-His374
Host
Human
Theoretical MW (kDa)
38
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 4.0 g/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CADM1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CADM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CADM1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CADM1 Antibody, hFc Tag with the EC50 of 4.0 g/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CADM1
Entrez GeneID
23705Protein Accession#
Q9BY67-1Gene Name
CADM1
Gene Alias
BL2, DKFZp686F1789, IGSF4, IGSF4A, MGC149785, MGC51880, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1
Gene Description
cell adhesion molecule 1
Omim ID
605686Gene Ontology
HyperlinkGene Summary
member 4|immunoglobulin superfamily
Other Designations
TSLC1/Nectin-like 2/IGSF4|immunoglobulin superfamily, member 4|immunoglobulin superfamily, member 4D|nectin-like 2|nectin-like protein 2|tumor suppressor in lung cancer 1
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Interactome
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Pathway
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Disease
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