GH1 (Bovine) Recombinant Protein
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Specification
Product Description
Bovine GH1 recombinant protein expressed in?Escherichia coli.
Sequence
AFPAMSLSGLFANAVLRAQHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETMPAPTGKNEAQQKSDLELLRISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDGTPRRGQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF
Host
Escherichia coli
Theoretical MW (kDa)
21.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 98% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
The activity determined by the PDFP13B9 cells transfected with rabbit GH receptors. Bovine GH forms a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
Storage Buffer
Protein (1 mg/mL) was lyophilized from a solution containing 4.5 mM NaHCO3, pH 8-9. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — GH
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