Products

Product Description

Fusion protein Cas9 nuclease expressed in an Escherichia coli with a N terminal end nuclear localization signal (NLS) and a C terminal end an EGFP and a 6x(His) sequence.

Host

Escherichia coli

Form

Liquid

Preparation Method

Escherichia coli expression system

Quality Control Testing

Cleavage assay

Lane 1: DNA + gRNA
Lane 2: marker
Lane 3: DNA + gRNA + NLS-Cas9-EGFP 100 ng
Lane 4: DNA + gRNA + NLS-Cas9-EGFP 50 ng

Recommend Usage

CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Storage Buffer

In 10 mM Tris, 300 mM NaCl, 1 mM DTT, pH7.4 (50% glycerol).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.