Trypsin, Irradiated
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Specification
Product Description
Lyophilized. Mycoplasma and extraneous virus free.
Biological function
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from inactive precursor zymogen, trypsinogen.
Host
Bovine
Form
Lyophilized
Preparation Method
Native protein chromatographically purified from Bovine Pancreas
Activity
>=180 TAME units per mg protein (at least 10,350 BAEE/3450 USP/NF units per mg protein). TAME Unit: One Unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.
Recommend Usage
Tissue dissociation (combined with other enzymes); Cell harvesting by trypsinization; Mitochondria isolation; in vitro studies of proteins; Various hemagglutination procedures; Sample preparation for flow cytometric DNA analysis; Tryptic mapping; Fingerprinting and sequencing work; Environmental monitoring; Subculturing cells; Cleavage fusion proteins; Generating glycopeptides from purified glycoproteins.
Storage Instruction
Store at 4°C.
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Applications
Enzyme Activity
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Gene Info — PRSS2
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Gene Info — PRSS1
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