AKR7A2 (Human) Recombinant Protein
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Specification
Product Description
Human AKR7A2 (NP_003680, 1 a.a. - 359 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSELEMLSAASRVVSRAAVHCALRSPPPEARALAMSRPPPPRVASVLGTMEMGRRMDAPASAAAVRAFLERGHTELDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYLHAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKALQAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLVAHECPNYFR
Host
Escherichia coli
Theoretical MW (kDa)
44
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 90% by SDS-PAGE
Activity
Specific activity: approximately 0.25-0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 umole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (1 mM DTT, 20% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — AKR7A2
Entrez GeneID
8574Protein Accession#
NP_003680Gene Name
AKR7A2
Gene Alias
AFAR, AFAR1, AFB1-AR1, AKR7
Gene Description
aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Omim ID
603418Gene Ontology
HyperlinkGene Summary
Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM
Other Designations
aflatoxin beta1 aldehyde reductase|aldo-keto reductase family 7, member A2|aldoketoreductase 7
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