Human immunodeficiency virus type 1 Nef monoclonal antibody, clone 3A2 (01-006)
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human immunodeficiency virus type 1 Nef.
Immunogen
Recombinant protein corresponding to human immunodeficiency virus type 1 (BRU) Nef.
Host
Mouse
Reactivity
Viruses
Specificity
Mapped to amino acids 171-190 (HGMDDPEREVLEWRFDSRLA - HIV-1 BRU). This antibody recognizes the rNef of HIV 1 subtypes A, B and C. It reacts with HIV1 Nef of BRU, IIIB, LAV and BH10 strains.
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines.
Kjerrstrom A, Hinkula J, Engstrom G, Ovod V, Krohn K, Benthin R, Wahren B.
Virology 2001 May; 284(1):46.
Application:WB-Tr, Human, HeLa cells.
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Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors.
Collings A, Pitkanen J, Strengell M, Tahtinen M, Lagerstedt A, Hakkarainen K, Ovod V, Sutter G, Ustav M, Ustav E, Mannik A, Ranki A, Peterson P, Krohn K.
Vaccine 1999 Oct; 18(5-6):460.
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Regulation of HIV-1 infection in astrocytes: expression of Nef, TNF-alpha and IL-6 is enhanced in coculture of astrocytes with macrophages.
Fiala M, Rhodes RH, Shapshak P, Nagano I, Martinez-Maza O, Diagne A, Baldwin G, Graves M.
J Neurovirol 1996 Jun; 2(3):158.
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Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines.
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