Polyoma virus, Medium T antigen monoclonal antibody, clone PyMT
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Specification
Product Description
Rat monoclonal antibody raised against polyoma virus, Medium T antigen.
Immunogen
Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
Host
Rat
Reactivity
Viruses
Specificity
Polyoma virus.
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b
Recommend Usage
Immunocytochemistry (1:10-1:2000)
Immunofluorescence (1:10-1:2000)
Immunoprecipitation (1:10-1:500)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-Glycine, 0.15 M NaCl (0.05% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Publication Reference
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Monoclonal antibodies against polyoma virus tumor antigens.
Dilworth SM, Griffin BE.
PNAS 1982 Feb; 79(4):1059.
Application:IP, Mouse, 3T6 cells.
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Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen.
Dilworth SM.
The EMBO Journal 1982 Jan; 1(11):1319.
Application:IP, KA, Mouse, 3T6 cells, Recombinant proteins.
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Monoclonal antibodies against polyoma virus tumor antigens.
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