CEA monoclonal antibody, clone PARLAM4
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Specification
Product Description
Mouse monoclonal antibody raised against native CEA.
Immunogen
Native purified CEA from membrane of human colon carcinoma cells.
Host
Mouse
Reactivity
Human
Specificity
This monoclonal antibody is reactive with human carcinoembryonic antigen (CEA), a tumor associated antigen with oncofetal characteristics. Immunoblotting - antibody reacts with a single band of 180 kD. In immunohistochemistry on tissue sections: no reactivity with granulocytes, bile canaliculi, alveolar epithelium. No crossreactivities have been observed. Despite this lack of specificity, immunohistochemical detection of CEA is frequently used for the histopathologial diagnosis of human tumors. Most polyclonal, non purified, CEA antisera show cross reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross reacting antigen 1/11 (NCA). Our monoclonal CEA antibody does not show cross reactivity neither with BGP nor with NCA.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:5-1:10)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-HCl (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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