Nefm monoclonal antibody, clone 3H11
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More Files
- More Functions
Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system. The lane show blots of this material stainted with Nefm monoclonal antibody, clone 3H11 (Cat # MAB2310).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Nefm.
Immunogen
Recombinant protein corresponding to C-terminus of rat Nefm.
Host
Mouse
Theoretical MW (kDa)
145-170
Reactivity
Birds, Human, Mammals, Mouse
Specificity
Specifically recognizes the medium neurofilament subunit (~145-170 KDa).
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
Western Blot (1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
No additive
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system. The lane show blots of this material stainted with Nefm monoclonal antibody, clone 3H11 (Cat # MAB2310).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — Nefm
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Publication Reference
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL.
FASEB Journal 2002 Apr; 16(8):869.
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A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.
Harris J, Ayyub C, Shaw G.
Journal of Neuroscience Research 1991 Sep; 30(1):47.
Application：WB, Fusion protein.
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
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